The Research Laboratory of Pulivarthi Rao, PhD

About the Research Laboratory of Pulivarthi Rao, PhD

The main goal of Dr. Pulivarthi Rao’s laboratory is the analysis of the genetic basis of deregulation of cellular proliferation and differentiation associated with malignant transformation.

The laboratory staff investigates several tumor types, most notably osteosarcoma and multiple myeloma. A wide range of functional, comparative and cytogenetic techniques is utilized in these studies, which include conventional and molecular cytogenetics- FISH, comparative genomic hybridization (CGH), multicolor spectral karyotyping (SKY), and array CGH. The laboratory emphasizes the identification of oncogenes from the amplified chromosomal regions and genomic insatbility in osteosarcoma. His laboratory is also focusing on the development of molecular cytogenetic techniques for the analysis of chromosomal aberrations in murine models of human cancers (Comparative Cancer Cytogenetics).

Dr. Pulivarthi Rao’s lab is part of the Cancer Genetics and Genomics Program, and the Sarcoma Research Labs.

Laboratory Members

Principal Investigator
Pulivarthi Rao, PhD

Research Assistant
Yi-Jue Zhao

Methods

FISH by indirect labeling
Direct Labeling for FISH
Paraffin FISH
Protocol for Q Fish
Preparation of human lymphocyte metaphase chromosomes
CGH Protocol
Multicolor Spectral Karyotyping

Links

Mouse Nomenclature
Human Gene Nomenclature Guidelines
Molecular Cytogenetics Resources
SKY/m-FISH and CGH database
Cancer genetics
NCBI